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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
Mouse Microglial Cell Line, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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mouse microglial cells  (CLS Cell Lines Service GmbH)
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in <t>microglial</t> <t>BV2</t> cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.
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In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in microglial BV2 cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: bioRxiv

Article Title: Olfactory proteomics reveals the capacity of the HDAC1 inhibitor pyroxamide to halt the α-synuclein preformed fibrils-induced damage in nasal epithelial, microglial and dopaminergic neuronal cell lines

doi: 10.1101/2025.10.02.679944

Figure Lengend Snippet: In vitro experiments in dopaminergic MN9D cells representing the survival effect of α-syn PFFs after 6h of pyroxamide pre-treatment (A). Survival effect of α-syn PFFs after 3 and 6 hours of pyroxamide pre-treatment in olfactory epithelial T9243 cells (B). Survival effect of α-syn PFFs after 3 hours of pyroxamide pre-treatment in microglial BV2 cells upon α-syn PFFs-induced damage (C). Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Similar conditions were used for the Dopaminergic Neuronal Cell Line (MN9D; Merck Millipore, SCC281), and the Mouse Microglial Cell Line (BV2; Cytion, 305156), seeded at 1 × 10^4 cells/well in 96-well plates.

Techniques: In Vitro

Survival analysis associated to pyroxamide pre-treatment 3 hours in dopaminergic neuronal (A) and microglial cells (B) upon oxidative stress conditions. Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01.

Journal: bioRxiv

Article Title: Olfactory proteomics reveals the capacity of the HDAC1 inhibitor pyroxamide to halt the α-synuclein preformed fibrils-induced damage in nasal epithelial, microglial and dopaminergic neuronal cell lines

doi: 10.1101/2025.10.02.679944

Figure Lengend Snippet: Survival analysis associated to pyroxamide pre-treatment 3 hours in dopaminergic neuronal (A) and microglial cells (B) upon oxidative stress conditions. Data were analyzed using one-way ANOVA. * indicates p < 0.05; ** p < 0.01.

Article Snippet: Similar conditions were used for the Dopaminergic Neuronal Cell Line (MN9D; Merck Millipore, SCC281), and the Mouse Microglial Cell Line (BV2; Cytion, 305156), seeded at 1 × 10^4 cells/well in 96-well plates.

Techniques: